Dataset Information

Gene expression profiling of CD8⁺ T cells induced by ovarian cancer cells suggests a possible mechanism for CD8⁺ Treg cell production.

ABSTRACT:

Objectives

The aim of this study was to investigate a possible mechanism of CD8⁺ regulatory T-cell (Treg) production in an ovarian cancer (OC) microenvironment.

Materials and methods

Agilent microarray was used to detect changes in gene expression between CD8⁺ T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line. QRT-PCR was performed to determine glycolysis gene expression in CD8⁺ T cells from a transwell culturing system and OC patients. We also detected protein levels of glycolysis-related genes using Western blot analysis.

Results

Comparing gene expression profiles revealed significant differences in expression levels of 1420 genes, of which 246 were up-regulated and 1174 were down-regulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that biological processes altered in CD8⁺ Treg are particularly associated with energy metabolism. CD8⁺ Treg cells induced by co-culture with SKOV3 had lower glycolysis gene expression compared to CD8⁺ T cells cultured alone. Glycolysis gene expression was also decreased in the CD8⁺ T cells of OC patients.

Conclusions

These findings provide a comprehensive bioinformatics analysis of DEGs in CD8⁺ T cells cultured with and without SKOV3 and suggests that metabolic processes may be a possible mechanism for CD8⁺ Treg induction.

SUBMITTER: Wu M

PROVIDER: S-EPMC6496584 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Publications

Gene expression profiling of CD8<sup>+</sup> T cells induced by ovarian cancer cells suggests a possible mechanism for CD8<sup>+</sup> Treg cell production.

Wu Meng M Lou Jianfang J Zhang Shuping S Chen Xian X Huang Lei L Sun Ruihong R Huang Peijun P Pan Shiyang S Wang Fang F

Cell proliferation 20160919 6

<h4>Objectives</h4>The aim of this study was to investigate a possible mechanism of CD8<sup>+</sup> regulatory T-cell (Treg) production in an ovarian cancer (OC) microenvironment.<h4>Materials and methods</h4>Agilent microarray was used to detect changes in gene expression between CD8<sup>+</sup> T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line. QRT-PCR was performed to determine glycolysis gene expression in CD8<sup>+</sup> T cells from a transwell culturing system a ...[more]

PMID: 27641758

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Project description:The pig has the potential to become a leading research model for human diseases, pharmacological and transplantation studies. Since there are many similarities between humans and pigs, especially concerning anatomy, physiology and metabolism, there is necessity for a better understanding of the porcine immune system. In adaptive immunity, cytotoxic T lymphocytes (CTLs) are essential for host defense. However, most data on CTLs come from studies in mice, non-human primates and humans, while detailed information about porcine CD8+ CTLs is still sparse. Aim of this study was to analyze transcriptomes of three subsets of porcine CD8β+ T-cell subsets by using next-generation sequencing technology. Specifically, we described transcriptional profiles of subsets defined by their CD11a/CD27 expression pattern, postulated as naïve (CD8β+CD27+CD11alow), intermediate differentiated (CD8β+CD27dimCD11a+), and terminally differentiated cells (CD8β+CD27-CD11ahigh). Cells were analyzed in ex vivo condition as well as upon in vitro stimulation with concanavalin A (ConA) and PMA/ionomycin. Our analyses show that the highest number of differentially expressed genes was identified between naïve and terminally differentiated CD8+ T-cell subsets, underlining their difference in gene expression signature and respective differentiation stages. Moreover, genes related to early (IL7-R, CCR7, SELL, TCF7, LEF1, BACH2, SATB1, ZEB1 and BCL2) and late (KLRG1, TBX21, PRDM1, CX3CR1, ZEB2, ZNF683, BATF, EZH2 and ID2) stages of CD8+ T-cell differentiation were highly expressed in the naïve and terminally differentiated CD8+ T-cell subsets, respectively. Intermediate differentiated CD8+ T-cell subsets shared a more comparable gene expression profile associated with later stages of T-cell differentiation. Genes associated with cytolytic activity (GNLY, PRF1, GZMB, FASL, IFNG and TNF) were highly expressed in terminally and intermediate differentiated CD8+ T-cell subsets, while naïve CD8+ T cells lacked expression even after in vitro stimulation. Overall, PMA/ionomycin stimulation induced much stronger upregulation of genes compared to stimulation with ConA. Taken together, we provided comprehensive results showing transcriptional profiles of three differentiation stages of porcine CD8+ T-cell subsets. In addition, our study provides a powerful toolbox for the identification of candidate markers to characterize porcine immune cell subsets in more detail.

Dataset Information

Gene expression profiling of CD8⁺ T cells induced by ovarian cancer cells suggests a possible mechanism for CD8⁺ Treg cell production.

Objectives

Materials and methods

Results

Conclusions

Publications

Gene expression profiling of CD8<sup>+</sup> T cells induced by ovarian cancer cells suggests a possible mechanism for CD8<sup>+</sup> Treg cell production.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Gene expression profiling of CD8+ T cells induced by ovarian cancer cells suggests a possible mechanism for CD8+ Treg cell production.

Objectives

Materials and methods

Results

Conclusions

Publications

Gene expression profiling of CD8<sup>+</sup> T cells induced by ovarian cancer cells suggests a possible mechanism for CD8<sup>+</sup> Treg cell production.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Gene expression profiling of CD8⁺ T cells induced by ovarian cancer cells suggests a possible mechanism for CD8⁺ Treg cell production.