Dataset Information

Contribution of pancreatic ?-cell function to insulin sensitivity and glycemic variability in patients with type 1 diabetes.

ABSTRACT:

Aims/introduction

To evaluate the contribution of pancreatic ?-cell function to the dawn phenomenon, insulin sensitivity, hepatic glucose uptake and glycemic variability in patients with type 1 diabetes.

Materials and methods

In 40 patients with type 1 diabetes, arginine stimulation tests were carried out, and the area under the curve (AUC) of glucagon was measured using radioimmunoassays (AUC_glc _RIA ) and enzyme-linked immunosorbent assays (AUC_glc _ELISA ). The ratio of the insulin dose delivered by an artificial pancreas to maintain euglycemia between 04.00 and 08.00 hours or between 00.00 and 04.00 hours was measured as the dawn index. The glucose infusion rate and hepatic glucose uptake were measured using hyperinsulinemic euglycemic clamp and clamp oral glucose loading tests. Glycemic variability in 96 h was measured by continuous glucose monitoring.

Results

The median dawn index (1.7, interquartile range 1.0-2.8) was not correlated with AUC_glc _RIA (R² = 0.03, P = 0.39) or AUC_glc _ELISA (R² = 0.04, P = 0.32). The median glucose infusion rate (7.3 mg/kg/min, interquartile range 6.4-9.2 mg/kg/min) was significantly correlated with AUC_glc _RIA (R² = 0.20, P = 0.02) and AUC_glc _ELISA (R² = 0.21, P = 0.02). The median hepatic glucose uptake (65.3%, interquartile range 40.0-87.3%) was not correlated with AUC_glc _RIA (R² = 0.07, P = 0.26) or AUC_glc _ELISA (R² = 0.26, P = 0.79). The standard deviation of glucose levels measured by continuous glucose monitoring was significantly correlated with AUC_glc _RIA (R² = 0.11, P = 0.049), but not with AUC_glc _ELISA (R² = 0.01, P = 0.75).

Conclusions

Pancreatic ?-cell function contributed to insulin sensitivity in patients with type 1 diabetes.

SUBMITTER: Takahashi N

PROVIDER: S-EPMC6497601 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Contribution of pancreatic α-cell function to insulin sensitivity and glycemic variability in patients with type 1 diabetes.

Takahashi Nobuyuki N Chujo Daisuke D Kajio Hiroshi H Ueki Kohjiro K

Journal of diabetes investigation 20181030 3

<h4>Aims/introduction</h4>To evaluate the contribution of pancreatic α-cell function to the dawn phenomenon, insulin sensitivity, hepatic glucose uptake and glycemic variability in patients with type 1 diabetes.<h4>Materials and methods</h4>In 40 patients with type 1 diabetes, arginine stimulation tests were carried out, and the area under the curve (AUC) of glucagon was measured using radioimmunoassays (AUC<sub>glc</sub> <sub>RIA</sub> ) and enzyme-linked immunosorbent assays (AUC<sub>glc</sub> ...[more]

PMID: 30290079

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