Unknown

Dataset Information

0

Genetic analysis of Nicotiana pollen-part mutants is consistent with the presence of an S-ribonuclease inhibitor at the S locus.


ABSTRACT: Self-incompatibility (SI) is a genetic mechanism that restricts inbreeding in flowering plants. In the nightshade family (Solanaceae) SI is controlled by a single multiallelic S locus. Pollen rejection in this system requires the interaction of two S locus products: a stylar (S)-RNase and its pollen counterpart (pollen S). pollen S has not yet been cloned. Our understanding of how this gene functions comes from studies of plants with mutations that affect the pollen but not the stylar SI response (pollen-part mutations). These mutations are frequently associated with duplicated S alleles, but the absence of an obvious additional allele in some plants suggests pollen S can also be deleted. We studied Nicotiana alata plants with an additional S allele and show that duplication causes a pollen-part mutation in several different genetic backgrounds. Inheritance of the duplication was consistent with a competitive interaction model in which any two nonmatching S alleles cause a breakdown of SI when present in the same pollen grain. We also examined plants with presumed deletions of pollen S and found that they instead have duplications that included pollen S but not the S-RNase gene. This finding is consistent with a bipartite structure for the S locus. The absence of pollen S deletions in this study and perhaps other studies suggests that pollen S might be required for pollen viability, possibly because its product acts as an S-RNase inhibitor.

SUBMITTER: Golz JF 

PROVIDER: S-EPMC65036 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Genetic analysis of Nicotiana pollen-part mutants is consistent with the presence of an S-ribonuclease inhibitor at the S locus.

Golz J F JF   Oh H Y HY   Su V V   Kusaba M M   Newbigin E E  

Proceedings of the National Academy of Sciences of the United States of America 20011201 26


Self-incompatibility (SI) is a genetic mechanism that restricts inbreeding in flowering plants. In the nightshade family (Solanaceae) SI is controlled by a single multiallelic S locus. Pollen rejection in this system requires the interaction of two S locus products: a stylar (S)-RNase and its pollen counterpart (pollen S). pollen S has not yet been cloned. Our understanding of how this gene functions comes from studies of plants with mutations that affect the pollen but not the stylar SI respons  ...[more]

Similar Datasets

| S-EPMC5545845 | biostudies-literature
| S-EPMC2219507 | biostudies-literature
| S-EPMC8539413 | biostudies-literature
| S-EPMC2818677 | biostudies-literature
| S-EPMC1993901 | biostudies-literature
2021-06-26 | GSE153474 | GEO
| S-EPMC2677630 | biostudies-literature
| S-EPMC4285803 | biostudies-other
| S-EPMC7469187 | biostudies-literature
2018-08-13 | PXD009184 | Pride