Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC6504903 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Chen Jingjing J Gardner Jennifer M JM Yu Zulin Z Smith Sarah E SE McKinney Sean S Slaughter Brian D BD Unruh Jay R JR Jaspersen Sue L SL
The Journal of cell biology 20190312 5
Bipolar spindle formation in yeast requires insertion of centrosomes (known as spindle pole bodies [SPBs]) into fenestrated regions of the nuclear envelope (NE). Using structured illumination microscopy and bimolecular fluorescence complementation, we map protein distribution at SPB fenestrae and interrogate protein-protein interactions with high spatial resolution. We find that the Sad1-UNC-84 (SUN) protein Mps3 forms a ring-like structure around the SPB, similar to toroids seen for components ...[more]