Dataset Information

Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture.

ABSTRACT: Linker of nucleoskeleton and cytoskeleton (LINC) complexes spanning the nuclear envelope (NE) contribute to nucleocytoskeletal force transduction. A few NE proteins have been found to regulate the LINC complex. In this study, we identify one, Kuduk (Kud), which can reside at the outer nuclear membrane and is required for the development of Drosophila melanogaster ovarian follicles and NE morphology of myonuclei. Kud associates with LINC complex components in an evolutionarily conserved manner. Loss of Kud increases the level but impairs functioning of the LINC complex. Overexpression of Kud suppresses NE targeting of cytoskeleton-free LINC complexes. Thus, Kud acts as a quality control mechanism for LINC-mediated nucleocytoskeletal connections. Genetic data indicate that Kud also functions independently of the LINC complex. Overexpression of the human orthologue TMEM258 in Drosophila proved functional conservation. These findings expand our understanding of the regulation of LINC complexes and NE architecture.

SUBMITTER: Ding ZY

PROVIDER: S-EPMC5584142 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture.

Ding Zhao-Ying ZY Wang Ying-Hsuan YH Huang Yu-Cheng YC Lee Myong-Chol MC Tseng Min-Jen MJ Chi Ya-Hui YH Huang Min-Lang ML

The Journal of cell biology 20170717 9

Linker of nucleoskeleton and cytoskeleton (LINC) complexes spanning the nuclear envelope (NE) contribute to nucleocytoskeletal force transduction. A few NE proteins have been found to regulate the LINC complex. In this study, we identify one, Kuduk (Kud), which can reside at the outer nuclear membrane and is required for the development of <i>Drosophila melanogaster</i> ovarian follicles and NE morphology of myonuclei. Kud associates with LINC complex components in an evolutionarily conserved ma ...[more]

PMID: 28716842

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Project description:BACKGROUND: DYT1 dystonia is an autosomal dominant neurological condition caused by a mutation that removes a single glutamic acid residue (?E) from the torsinA (torA) AAA+ protein. TorA appears to possess a nuclear envelope (NE) localized activity that requires Lamina-Associated-Polypeptide 1 (LAP1), which is an inner nuclear membrane localized torA-binding partner. Although hypoactive, the DYT1 dystonia torA-?E isoform often concentrates in the NE, suggesting that torA-?E also interacts with an NE-localized binding partner. RESULTS: We confirm that NE-localized torA-?E does not co-immunoprecipitate with LAP1, and find that torA-?E continues to concentrate in the NE of cells that lack LAP1. Instead, we find that variability in torA-?E localization correlates with the presence of the SUN-domain and Nesprin proteins that assemble into the LINC complex. We also find that siRNA depletion of SUN1, but not other LINC complex components, removes torA-?E from the NE. In contrast, the LAP1-dependent NE-accumulation of an ATP-locked torA mutant is unaffected by loss of LINC complex proteins. This SUN1 dependent torA-?E localization requires the torA membrane association domain, as well as a putative substrate-interaction residue, Y147, neither of which are required for torA interaction with LAP1. We also find that mutation of these motifs, or depletion of SUN1, decreases the amount of torA-WT that colocalizes with NE markers, indicating that each also underlies a normal NE-localized torA binding interaction. CONCLUSIONS: These data suggest that the disease causing ?E mutation promotes an association between torA and SUN1 that is distinct to the interaction between LAP1 and ATP-bound torA. This evidence for two NE-localized binding partners suggests that torA may act on multiple substrates and/or possesses regulatory co-factor partners. In addition, finding that the DYT1 mutation causes abnormal association with SUN1 implicates LINC complex dysfunction in DYT1 dystonia pathogenesis, and suggests a gain-of-function activity contributes to this dominantly inherited disease.

Dataset Information

Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture.

Publications

Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture.

Similar Datasets

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