Ultrashort echo time magnetic resonance imaging (UTE-MRI) of cortical bone correlates well with histomorphometric assessment of bone microstructure.

ABSTRACT: Ultrashort echo time magnetic resonance imaging (UTE-MRI) techniques have been increasingly used to assess cortical bone microstructure. High resolution micro computed tomography (?CT) is routinely employed for validating the MRI-based assessments. However, water protons in cortical bone may reside in micropores smaller than the detectable size ranges by ?CT. The goal of this study was to evaluate the upper limit of UTE-MRI and compare its efficacy to ?CT at determining bone porosity ex vivo. This study investigated the correlations between UTE-MRI based quantifications and histomorphometric measures of bone porosity that cover all pores larger than 1??m. Anterior tibial midshaft specimens from eleven donors (51?±?16?years old, 6 males, 5 females) were scanned on a clinical 3?T-MRI using UTE magnetization transfer (UTE-MT, three power levels and five frequency offsets) and UTE-T2* sequences. Two-pool MT modeling and bi-component exponential T2* fitting were performed on the MRI datasets. Specimens were then scanned by ?CT at 9??m voxel size. Histomorphometry was performed on hematoxylin and eosin (H&E) stained slides imaged at submicron resolution. Macromolecular fraction from MT modeling, bi-component T2* fractions, and short component T2* showed strong correlations (R?>?0.7, p?40??m) porosities as well as with ?CT-based porosity. UTE-MRI could also assess small pores variations with moderate correlations (R?>?0.5, p?

SUBMITTER: Jerban S 

PROVIDER: S-EPMC6504977 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature