High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription.

ABSTRACT: Recent studies on targeted gene integrations in bacteria have demonstrated that chromosomal location can substantially affect a gene's expression level. However, these studies have only provided information on a small number of sites. To measure position effects on transcriptional propensity at high resolution across the genome, we built and analyzed a library of over 144,000 genome-integrated, standardized reporters in a single mixed population of Escherichia coli. We observed more than 20-fold variations in transcriptional propensity across the genome when the length of the chromosome was binned into broad 4 kbp regions; greater variability was observed over smaller regions. Our data reveal peaks of high transcriptional propensity centered on ribosomal RNA operons and core metabolic gene

SUBMITTER: Scholz SA 

PROVIDER: S-EPMC6508686 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature