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CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics.

ABSTRACT: High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptomes using a variety of cell types, including long-term tracking of cell engraftment and differentiation in vivo. Together, this presents CellTag Indexing as a broadly applicable genetic multiplexing tool that is complementary with existing single-cell technologies.

SUBMITTER: Guo C

PROVIDER: S-EPMC6509836 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics.

Guo Chuner C Kong Wenjun W Kamimoto Kenji K Rivera-Gonzalez Guillermo C GC Yang Xue X Kirita Yuhei Y Morris Samantha A SA

Genome biology 20190509 1

High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptomes usi ...[more]

PMID: 31072405

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