Dataset Information
TROSY pulse sequence for simultaneous measurement of the 15N R1 and {1H}-15N NOE in deuterated proteins.
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ABSTRACT:
SUBMITTER: O'Brien PA
PROVIDER: S-EPMC6510663 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Publications
TROSY pulse sequence for simultaneous measurement of the <sup>15</sup>N R<sub>1</sub> and {<sup>1</sup>H}-<sup>15</sup>N NOE in deuterated proteins.
Journal of biomolecular NMR 20180416 4
A TROSY-based NMR experiment is described for simultaneous measurement of the <sup>15</sup>N longitudinal relaxation rate constant R<sub>1</sub> and the {<sup>1</sup>H}-<sup>15</sup>N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges <sup>1</sup>H and <sup>15</sup>N magnetizations. The accuracy of the proposed technique is validated by comparison to independent measurements of both relaxation parameters ...[more]