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Evaluation of intervertebral disc regeneration with injection of mesenchymal stem cells encapsulated in PEGDA-microcryogel delivery system using quantitative T2 mapping: a study in canines.

ABSTRACT: Intervertebral disc degeneration (IDD), the primary cause of low back pain, is still a great challenge to spinal surgeons and clinicians. T2 mapping, a biochemical magnetic resonance imaging (MRI) technique to calculate relaxation time, has the potential to offer a quantitative assessment of IDD. The aim of the study was to evaluate the regenerative effects of adipose-derived mesenchymal stem cells (MSCs) encapsulated in PEGDA-microcryogels (PMs) reinforced alginate hydrogel (AH) on the degenerative intervertebral disc (IVD) in a canine model using T2 mapping. Four degeneration-induced IVDs (L3-L4 to L6-L7) of 12 adult beagle dogs were injected with phosphate-buffered saline (PBS), MSCs, AH-PMs, and MSCs-laden AH-PMs, respectively. The intact IVD L7-S1 served as the normal control. IVD height change on plain radiograph, Pfirrmann grade and T2 relaxation time on MRI, histological change, and extracellular matrix (ECM)-associated proteins were evaluated during the 24-week follow-up period. Injection of MSCs-laden AH-PMs had the most satisfactory effects, having less decrease of IVD height, lower Pfirrmann grade, milder histological change, and longer T2 relaxation time (P < 0.05). T2 relaxation time was positively correlated with ECM content (proteoglycan: r = 0.85, P < 0.001; collagen II: r = 0.79, P < 0.001) and IVD height (r = 0.81, P < 0.001), but negatively correlated with Pfirrmann grade and histological grade (rho = -0.86, P < 0.001; rho = -0.95, P < 0.001). These results suggest that T2 mapping has the potential to quantitatively evaluate the repairing effects of cell-based engineering treatments on IDD for a long-term follow-up.

SUBMITTER: Ying J

PROVIDER: S-EPMC6511749 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Evaluation of intervertebral disc regeneration with injection of mesenchymal stem cells encapsulated in PEGDA-microcryogel delivery system using quantitative T2 mapping: a study in canines.

Ying Jinwei J Han Zhihua Z Zeng Yang Y Du Yanan Y Pei Shishen S Su Linghao L Ruan Dike D Chen Chun C

American journal of translational research 20190415 4

Intervertebral disc degeneration (IDD), the primary cause of low back pain, is still a great challenge to spinal surgeons and clinicians. T2 mapping, a biochemical magnetic resonance imaging (MRI) technique to calculate relaxation time, has the potential to offer a quantitative assessment of IDD. The aim of the study was to evaluate the regenerative effects of adipose-derived mesenchymal stem cells (MSCs) encapsulated in PEGDA-microcryogels (PMs) reinforced alginate hydrogel (AH) on the degenera ...[more]

PMID: 31105815

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Project description:BackgroundLow back pain (LPB) is the main cause of disability worldwide with enormous socioeconomic burdens. A major cause of LBP is intervertebral disc degeneration (IDD): a chronic, progressive process associated with exhaustion of the resident cell population, tissue inflammation, degradation of the extracellular matrix and dehydration of the nucleus pulposus. Eventually, IDD may lead to serious sequelae including chronic LBP, disc herniation, segmental instability, and spinal stenosis, which may require invasive surgical interventions. However, no treatment is actually able to directly tackle IDD and hamper the degenerative process. In the last decade, the intradiscal injection of stem cells is raising as a promising approach to regenerate the intervertebral disc. This review aims to describe the rationale behind a regenerative stem cell therapy for IDD as well as the effect of stem cells following their implantation in the disc environment according to preclinical studies. Furthermore, actual clinical evidence and ongoing trials will be discussed, taking into account the future perspective and current limitations of this cutting-edge therapy.MethodsA literature analysis was performed for this narrative review. A database search of PubMed, Scopus and ClinicalTrials.gov was conducted using "stem cells" combined with "intervertebral disc", "degeneration" and "regeneration" without exclusion based on publication date. Articles were firstly screened on a title-abstract basis and, subsequently, full-text were reviewed. Both preclinical and clinical studies have been included.ResultsThe database search yielded recent publications from which the narrative review was completed.ConclusionsBased on available evidence, intradiscal stem cell therapy has provided encouraging results in terms of regenerative effects and reduction of LBP. However, multicenter, prospective randomized trials are needed in order confirm the safety, efficacy and applicability of such a promising treatment.

Dataset Information

Evaluation of intervertebral disc regeneration with injection of mesenchymal stem cells encapsulated in PEGDA-microcryogel delivery system using quantitative T2 mapping: a study in canines.

Publications

Evaluation of intervertebral disc regeneration with injection of mesenchymal stem cells encapsulated in PEGDA-microcryogel delivery system using quantitative T2 mapping: a study in canines.

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