Dataset Information

Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.

ABSTRACT:

Aim

To study the effects of exercise on lysosomal functions.

Methods

Mouse exercise model was established and wheel running was scheduled as 18 rpm (14:00-17:00), 5 d/wk, for 8 weeks. Mice were injected EX527 to inhibit SIRT1 activity. The protein level was assayed with Western blot and immunofluorescence histochemistry. The transmission electron microscopic examination was used to show the structure of lysosome and mitochondria.

Results

Exercise promoted the nuclear translocation of TFEB in the cortex which upregulated the transcription of genes associated with autophagy and lysosome. Exercise directly activated autophagy/lysosome system via up-regulating of AMPK-SIRT1 signaling. The SIRT1 inhibitor EX527 decreased TFEB regulated gene transcription but had little effect on the nuclear translocation of TFEB. In addition, long-term exercise showed more significant effects on activation of lysosomes biogenesis compared with the short-term exercise and trehalose, a classical autophagy activator in the mTOR-independent pathway.

Conclusion

Running exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.

SUBMITTER: Huang J

PROVIDER: S-EPMC6515701 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Publications

Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.

Huang Jun J Wang Xue X Zhu Yi Y Li Zhe Z Zhu Yu-Ting YT Wu Jun-Chao JC Qin Zheng-Hong ZH Xiang Min M Lin Fang F

CNS neuroscience & therapeutics 20190312 6

<h4>Aim</h4>To study the effects of exercise on lysosomal functions.<h4>Methods</h4>Mouse exercise model was established and wheel running was scheduled as 18 rpm (14:00-17:00), 5 d/wk, for 8 weeks. Mice were injected EX527 to inhibit SIRT1 activity. The protein level was assayed with Western blot and immunofluorescence histochemistry. The transmission electron microscopic examination was used to show the structure of lysosome and mitochondria.<h4>Results</h4>Exercise promoted the nuclear transl ...[more]

PMID: 30864262

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Dataset Information

Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.

Aim

Methods

Results

Conclusion

Publications

Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway.

Similar Datasets

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