Dataset Information

ASSOCIATION OF THE HUMAN PPAR?2 PRO12ALA POLYMORPHISM WITH OBESITY IN A POPULATION FROM TURKEY.

ABSTRACT:

Background

There have been a number of reports on the relationship between the PPAR?2 Pro12Ala genotype and the development of obesity.

Objective

A case-control survey was designed to investigate the potential association between a Pro12Ala polymorphism in the PPAR?2 gene and obesity and/or obesity-related phenotypes in a population from Turkey.

Materials and methods

The polymerase chain reaction and restriction enzyme digestion were used to genotype the Pro12Ala polymorphism of the PPAR?2 gene in 149 unrelated obese and 105 non-obese control subjects from Turkey. The data were analyzed statistically.

Results

We found that the overall minor allele frequency was 0.12 in cases and 0.095 in controls. In terms of genotype distribution and allele frequencies among the cases versus controls in the population studied, only the gender-stratified analysis revealed a significantly higher frequency of Pro/Ala genotype within males. The polymorphism was associated with significantly higher weight, height, waist circumference, central adiposity (waist-to-hip ratio, WHR), lean body weight as well as dry body weight, but not overall adiposity (total body fat percentage, TBF) in cases carrying Ala allele (Pro/Ala or Ala/Ala). However, in the subjects carrying Ala allele of the control group, WHR values were found significantly lower.

Conclusion

Our results showed that the Pro12Ala polymorphism in the PPAR?2 gene is associated with obesity in the studied adult population from Turkey. These data suggest that the Pro12Ala polymorphism in PPAR?2 may be a potential genetic risk factor for central obesity.

SUBMITTER: Sozen MA

PROVIDER: S-EPMC6516403 | biostudies-literature | 2018 Oct-Dec

REPOSITORIES: biostudies-literature

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Publications

ASSOCIATION OF THE HUMAN PPARγ2 PRO12ALA POLYMORPHISM WITH OBESITY IN A POPULATION FROM TURKEY.

Sözen M A MA Özcan M U MU Çıldır M M Doğru I H IH Aygök A G AG Balkan K Ü KÜ

Acta endocrinologica (Bucharest, Romania : 2005) 20181001 4

<h4>Background</h4>There have been a number of reports on the relationship between the PPARγ2 Pro12Ala genotype and the development of obesity.<h4>Objective</h4>A case-control survey was designed to investigate the potential association between a Pro12Ala polymorphism in the PPARγ2 gene and obesity and/or obesity-related phenotypes in a population from Turkey.<h4>Materials and methods</h4>The polymerase chain reaction and restriction enzyme digestion were used to genotype the Pro12Ala polymorphi ...[more]

PMID: 31149297

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Project description:The objective of this study was to verify the influence of the Pro12Ala polymorphism of the PPARγ2 gene in response of a training program on the body composition. Sixty-nine previously inactive men and women (32.8 ± 8.2 years) were genotyped and underwent a 12-week aerobic (running/walking) training program (3-5 sessions, 40 - 60 min per session, and intensity between the aerobic and anaerobic threshold) (experimental group n = 53) or were part of the control group (n = 16). They were tested for aerobic capacity (ergospirometry), body composition (DXA), abdomen, waist and hip circumferences and nutritional assessment before and 48 h after the experimental protocol. Two-way repeated measures ANOVA test was used to verify possible differences in variables between the experimental vs. control groups or Pro/Pro vs. Pro/Ala groups, and the Chi-squared test was used to verify the distribution of responders and non-responders according to genotype (p < 0.05). Frequencies of 75.5% Pro/Pro (n = 40) and 24.5% Pro/Ala (n = 13) were found, without any occurrence of the recessive homozygote. Body fat reduction was initially confirmed compared to a control group which did not exercise (n = 16; 29.1 ± 8.8 years), so that the exercise group obtained a reduction of -1.3 kg vs. -0.3 kg in the control group (p = 0.03). When they were divided by genotype, there were significant changes in fat mass (-1.3 ± 2.1 kg; p = 0.00), lean mass (0.6 ± 1.5 kg; p = 0.02), fat percentage (-1.3 ± 1.6; p = 0.00), waist circumference (-2.2 ± 2.9 cm; p = 0.00), abdomen circumference (-3.3 ± 3.6 cm; p = 0.00) and hip circumference (-2.7 ± 2.7 cm; p = 0.00) for Pro/Pro genotypes; and fat mass (-1.1 ± 1.7 kg; p = 0.04), fat percentage (-0.9 ± 1.5; p = 0.04), abdomen circumference (-3.9 ± 3.5 cm; p = 0.00) and hip circumference (-1.8 ± 1.8 cm; p = 0.00) for Pro/Ala genotypes, without any group interaction differences. The Chi squared test revealed no differences in the distribution of responders or non-responders according to genotype. It is concluded that an aerobic training program promotes weight loss, but the Pro12Ala polymorphism in the PPARγ2 gene does not influence the variability of aerobic-induced exercise weight loss.