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Titanium dioxide modified with silver by two methods for bactericidal applications.

ABSTRACT: Titanium dioxide (TiO₂) is a semiconductor material that exhibits antibacterial activity due to its photocatalytic properties under ultraviolet light. On the other hand, silver also exhibits strong antibacterial activity towards a wide range of microorganisms and TiO₂ with silver addition exhibits more efficient photocatalytic properties than unmodified TiO₂. In this work, TiO₂ nanoparticles were synthesized by the hydrothermal method and modified with silver by two different methods: wet impregnation (Ex situ) and In situ incorporation. The antimicrobial activity of TiO₂ nanoparticles synthesized and modified by both methods was evaluated against Escherichia coli and Staphylococcus aureus. The results showed that TiO₂ nanoparticles have anatase phase. Also, spherical morphology with a mean particle size around 10.6 nm was obtained. The presence of silver in the modified TiO₂ nanoparticles was confirmed by EDS and XPS. TiO₂ particles modified by the Ex situ method, showed a better bactericidal activity compared to the particles modified by In situ incorporation method and TiO₂ unmodified nanoparticles. This study demonstrated that both methods used to modify the titanium dioxide nanoparticles are effective as bactericidal materials and better results were found for the Ex situ method.

SUBMITTER: Durango-Giraldo G

PROVIDER: S-EPMC6522664 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Titanium dioxide modified with silver by two methods for bactericidal applications.

Durango-Giraldo G G Cardona A A Zapata Juan Felipe JF Santa Juan Felipe JF Buitrago-Sierra R R

Heliyon 20190514 5

"Titanium dioxide (TiO2) is a semiconductor material that exhibits antibacterial activity due to its photocatalytic properties under ultraviolet light. On the other hand, silver also exhibits strong antibacterial activity towards a wide range of microorganisms and TiO2 with silver addition exhibits more efficient photocatalytic properties than unmodified TiO2. In this work, TiO2 nanoparticles were synthesized by the hydrothermal method and modified wit ...[more]

PMID: 31193210

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Project description:Silver nanoparticles (AgNPs) have a strong antimicrobial activity against a variety of pathogenic bacteria. The killing mechanism of AgNPs involves direct physical membrane destruction and subsequent molecular damage from both AgNPs and released Ag+. Burkholderia pseudomallei is the causative agent of melioidosis, an endemic infectious disease primarily found in northern Australia and Southeast Asia. B. pseudomallei is intrinsically resistant to most common antibiotics. In this study, the antimicrobial activity and mechanism of AgNPs (10-20 nm) against B. pseudomallei were investigated. The MIC and MBC for nine B. pseudomallei strains ranged from 32-48 μg/mL and 96-128 μg/mL, respectively. Concentrations of AgNPs less than 256 μg/mL were not toxic to human red blood cells. AgNPs exhibited a two-phase mechanism: cell death induction and ROS induction. The first phase was a rapid killing step within 5 min, causing the direct damage of the cytoplasmic membrane of the bacterial cells, as observed by a time-kill assay and fluorescence microscopy. During the period of 5-30 min, the cell surface charge was rapidly neutralized from -8.73 and -7.74 to 2.85 and 2.94 mV in two isolates of B. pseudomallei, as revealed by zeta potential measurement. Energy-dispersive X-ray (EDX) spectroscopy showed the silver element deposited on the bacterial membrane, and TEM micrographs of the AgNP-treated B. pseudomallei cells showed severe membrane damage and cytosolic leakage at 1/5 MIC and cell bursting at MBC. During the killing effect the released Ag+ from AgNPs was only 3.9% from the starting AgNPs concentration as observed with ICP-OES experiment. In the second phase, the ROS induction occurred 1-4 hr after the AgNP treatment. Altogether, we provide direct kinetic evidence of the AgNPs killing mechanism, by which cell death is separable from the ROS induction and AgNPs mainly contributes in the killing action. AgNPs may be considered a potential candidate to develop a novel alternative agent for melioidosis treatment with fast action.

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Titanium dioxide modified with silver by two methods for bactericidal applications.

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Titanium dioxide modified with silver by two methods for bactericidal applications.

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