Unknown

Dataset Information

0

Division of Labor: Roles of Groucho and CtBP in Notch-Mediated Lateral Inhibition that Controls Intestinal Stem Cell Differentiation in Drosophila.


ABSTRACT: Intestinal stem cell (ISC) differentiation in the Drosophila midgut requires Delta/Notch-mediated lateral inhibition, which separates the fate of ISCs from differentiating enteroblasts (EBs). Although a canonical Notch signaling cascade is involved in the lateral inhibition, its regulation at the transcriptional level is still unclear. Here we show that the establishment of lateral inhibition between ISC-EB requires two evolutionarily conserved transcriptional co-repressors Groucho (Gro) and C-terminal binding protein (CtBP) that act differently. Gro functions in EBs with E(spl)-C proteins to suppress Delta expression, inhibit cell-cycle re-entry, and promote cell differentiation, whereas CtBP functions specifically in ISCs to mediate transcriptional repression of Su(H) targets and maintain ISC fate. Interestingly, several E(spl)-C genes are also expressed in ISCs that cooperate with Gro to inhibit cell proliferation. Collectively, our study demonstrates separable and cell-type-specific functions of Gro and CtBP in a lateral inhibition process that controls the proliferation and differentiation of tissue stem cells.

SUBMITTER: Guo X 

PROVIDER: S-EPMC6523041 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Division of Labor: Roles of Groucho and CtBP in Notch-Mediated Lateral Inhibition that Controls Intestinal Stem Cell Differentiation in Drosophila.

Guo Xingting X   Huang Huanwei H   Yang Ziqing Z   Cai Tao T   Xi Rongwen R  

Stem cell reports 20190411 5


Intestinal stem cell (ISC) differentiation in the Drosophila midgut requires Delta/Notch-mediated lateral inhibition, which separates the fate of ISCs from differentiating enteroblasts (EBs). Although a canonical Notch signaling cascade is involved in the lateral inhibition, its regulation at the transcriptional level is still unclear. Here we show that the establishment of lateral inhibition between ISC-EB requires two evolutionarily conserved transcriptional co-repressors Groucho (Gro) and C-t  ...[more]

Similar Datasets

2019-03-15 | GSE112331 | GEO
| S-EPMC3208554 | biostudies-literature
| S-EPMC3744027 | biostudies-literature
| S-EPMC3159971 | biostudies-literature
| S-EPMC8289079 | biostudies-literature
| S-EPMC3383230 | biostudies-literature
| S-EPMC6083221 | biostudies-literature
| S-EPMC8339073 | biostudies-literature
| S-EPMC6331042 | biostudies-literature
| S-EPMC5877992 | biostudies-literature