Dataset Information

Global Transcriptomic Profile Analysis of Genes Involved in Lignin Biosynthesis and Accumulation Induced by Boron Deficiency in Poplar Roots.

ABSTRACT: To uncover the transcriptomic mechanism of lignin accumulation caused by boron deficiency (BD), Nanlin895 (Populus × euramericana "Nanlin895") was subjected to control (CK, 0.25 mg·L-1) and BD (0 mg·L-1) treatments for 3 days. RNA-Seq was carried out to survey the expression patterns of the lignin-regulated biosynthetic genes in response to BD. The results showed that 5946 genes were identified as differentially expressed genes (DEGs), 2968 (44.2%) of which were upregulated and 3318 (55.8%) of which were downregulated in response to BD. Among them, the expression of lignin monomer biosynthetic (PAL, CCR, CAD, COMT, F5H, PER/LAC) and modulated genes, for example, transcription factors (MYBs) and hormone signal regulating genes (GIDs, histidine kinase 1, coronatine-insensitive protein 1), were upregulated, and some hormone signal regulating genes, such as AUXs and BR-related (sterol methyltransferases), were downregulated under BD treatment. There are also some genes that were screened as candidates for an association with wood formation, which will be used for the further analysis of the function of lignin formation. These results provide an important theoretical basis and reference data in plant for further research on the mechanism of lignin accumulation under BD.

SUBMITTER: Su WL

PROVIDER: S-EPMC6523340 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Global Transcriptomic Profile Analysis of Genes Involved in Lignin Biosynthesis and Accumulation Induced by Boron Deficiency in Poplar Roots.

Su Wan-Long WL Liu Na N Mei Li L Luo Jie J Zhu Yi-Jie YJ Liang Zhu Z

Biomolecules 20190419 4

To uncover the transcriptomic mechanism of lignin accumulation caused by boron deficiency (BD), Nanlin895 (Populus × euramericana "Nanlin895") was subjected to control (CK, 0.25 mg·L-1) and BD (0 mg·L-1) treatments for 3 days. RNA-Seq was carried out to survey the expression patterns of the lignin-regulated biosynthetic genes in response to BD. The results showed that 5946 genes were identified as differentially expressed genes (DEGs), 2968 (44.2%) of which we ...[more]

PMID: 31010161

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Project description:Boron (B) is an essential nutrient for normal growth of plants. Despite its low abundance in soils, B pollution and toxicity have been reported to increase in especially arid and semi-arid environments. Poplars are known to be tolerant species to B toxicity and accumulation. In the current study, physiological, transcriptomic and hormonal regulations behind B toxicity response were investigated comparatively in poplar species. Previously identified clones of Populus nigra (P.n) and Populus alba (P.a) having contrasting B accumulation and leaf B toxicity symptoms were treated with elevated soil B supply in a pot trial. The physiological results of treatment indicated better biomass growth, higher leaf chlorophyll content and more than three folds lower B accumulation in leaves of P.a compared to P.n. Microarray based transcriptomic analysis revealed 1902 and 1006 differentially regulated transcripts for the leaves and roots of P.a, respectively. Several transcripts responsible in salicylic acid (SA) production (salicylic acid binding protein 2) and SA dependent gene regulation (chitinases, proteases, lipases and protease inhibitors) were strongly upregulated specifically in P.a tissues under B toxicity. Furthermore, endogenous SA content in the roots and leaves of P.a increased with a soil B concentration-dependent manner while there was no significant alteration in the same hormone for P.n tissues under B toxicity. Therefore, increase in endogenous SA concentration was strongly attributed to lower B uptake and B toxicity tolerance in P.a. In addition to SA mediated gene regulation, genes responsible in external excretion process was also supposed to be functional in P.a for reduction of tissue B content under toxic conditions. On the other hand, transcriptome profiling of P.n under B toxicity revealed 1624 and 1419 altered transcripts for the leaves and roots, respectively. Specific induction in transcripts annotated to ATP binding cassette B type transporters, glutathione S transferases and metallochaperones in P.n were linked to internal excretion of excess B in the species that could be related with much higher B uptake from the roots, directional transport to the leaves and detoxification under toxic B conditions.

Dataset Information

Global Transcriptomic Profile Analysis of Genes Involved in Lignin Biosynthesis and Accumulation Induced by Boron Deficiency in Poplar Roots.

Publications

Global Transcriptomic Profile Analysis of Genes Involved in Lignin Biosynthesis and Accumulation Induced by Boron Deficiency in Poplar Roots.

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