Dataset Information

A Reversibly Responsive Fluorochromic Hydrogel Based on Lanthanide-Mannose Complex.

ABSTRACT: Fluorochromic materials that are dynamic in response to external stimuli are of great interest for the development of advanced sensors and luminescent materials. Herein, a design based on a lanthanide-containing polymeric hydrogel possessing characteristic emission of lanthanides (Eu and Tb) and showing response to stimuli of metal ions is reported. The fluorochromic hydrogel is prepared using a lanthanide-mannose complex in gelation matrix. The lanthanide-mannose complex shows tunable fluorescent emission in response to Fe²⁺, due to the inhibition of the "antenna effect" between metal ions and ligands upon stimulation. The fluorescent hydrogel shows reversible "On/Off" fluorochromic response to Fe²⁺/ethylenediaminetetraacetic acid (EDTA). Remarkably, the fluorescent hydrogel is proven nontoxic and biocompatible; and a proof-of-application as in situ 3D cell culture extracellular matrix with reversible fluorochromic "On/Off" switch upon Fe²⁺/EDTA is demonstrated. This reversibly responsive fluorochromic hydrogel demonstrates a way to fabricate smart optical materials, particularly for biological-related applications where reversible response is required.

SUBMITTER: Meng K

PROVIDER: S-EPMC6523369 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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A Reversibly Responsive Fluorochromic Hydrogel Based on Lanthanide-Mannose Complex.

Meng Ke K Yao Chi C Ma Qianmin Q Xue Zhaohui Z Du Yaping Y Liu Wenguang W Yang Dayong D

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20190313 10

Fluorochromic materials that are dynamic in response to external stimuli are of great interest for the development of advanced sensors and luminescent materials. Herein, a design based on a lanthanide-containing polymeric hydrogel possessing characteristic emission of lanthanides (Eu and Tb) and showing response to stimuli of metal ions is reported. The fluorochromic hydrogel is prepared using a lanthanide-mannose complex in gelation matrix. The lanthanide-mannose complex shows tunable fluoresce ...[more]

PMID: 31131192

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Project description:Nonmembrane-bound organelles that behave like liquid droplets are widespread among eukaryotic cells. Their dysregulation appears to be a critical step in several neurodegenerative conditions. Here, we report that tau protein, the primary constituent of Alzheimer neurofibrillary tangles, can form liquid droplets and therefore has the necessary biophysical properties to undergo liquid-liquid phase separation (LLPS) in cells. Consonant with the factors that induce LLPS, tau is an intrinsically disordered protein that complexes with RNA to form droplets. Uniquely, the pool of RNAs to which tau binds in living cells are tRNAs. This phase state of tau is held in an approximately 1:1 charge balance across the protein and the nucleic acid constituents, and can thus be maximal at different RNA:tau mass ratios, depending on the biopolymer constituents involved. This feature is characteristic of complex coacervation. We furthermore show that the LLPS process is directly and sensitively tuned by salt concentration and temperature, implying it is modulated by both electrostatic interactions between the involved protein and nucleic acid constituents, as well as net changes in entropy. Despite the high protein concentration within the complex coacervate phase, tau is locally freely tumbling and capable of diffusing through the droplet interior. In fact, tau in the condensed phase state does not reveal any immediate changes in local protein packing, local conformations and local protein dynamics from that of tau in the dilute solution state. In contrast, the population of aggregation-prone tau as induced by the complexation with heparin is accompanied by large changes in local tau conformations and irreversible aggregation. However, prolonged residency within the droplet state eventually results in the emergence of detectable β-sheet structures according to thioflavin-T assay. These findings suggest that the droplet state can incubate tau and predispose the protein toward the formation of insoluble fibrils.

Dataset Information

A Reversibly Responsive Fluorochromic Hydrogel Based on Lanthanide-Mannose Complex.

Publications

A Reversibly Responsive Fluorochromic Hydrogel Based on Lanthanide-Mannose Complex.

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