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Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats.

ABSTRACT: Background:Panax notoginseng (Burk.) F. H. Chen is one of the most common herbs in China. Because of its good efficacy and little adverse reaction, Panax notoginseng has been used widely to treat cardiovascular diseases (CVDs). Objective:To investigate effects of Panax notoginseng flower (PN-F) on rats with myocardial infarction (MI). Methods:The proximal left anterior descending coronary artery in rats was ligated to induce acute myocardial infarction. Then, animals were randomly assigned to four experimental groups: MI control group, Betaloc control group (with Betaloc 10?mg/kg/d), FD500 (low-dose) group (Panax notoginseng flower decoction 500?mg/kg, n=10), and FD1000 (high-dose) group (Panax notoginseng flower decoction 1000?mg/kg, n=10). Panax notoginseng flower decoction or Betaloc was orally administrated for two to four weeks before and after operation. Sham-operated group was used as a normal untreated group, in which animals were treated with double distilled water, once daily. HE (hematoxylin and eosin) staining, immunofluorescent assay, TUNEL assay, quantitative real-time PCR, and western blot analysis were, respectively, performed to observe morphology, count mean minimal vessels, investigate apoptotic cells, and record gene (HIF-1, VEGFA, and KDR) and protein (Bcl-2 and Bax) expressions. Results:Two weeks after MI, PN-F significantly enhanced capillary density in the border area of MI, decreased infarct size, improved minimal vessels, suppressed cell apoptosis, and enhanced expressions of genes (HIF-1, VEGFA, and KDR) and proteins (Bcl-2 and Bax). Conclusions:PN-F demonstrated a potential herb to treat rats with myocardial infarction through promoting angiogenesis and inhibition of apoptosis in the infarct area.

SUBMITTER: Zhou X

PROVIDER: S-EPMC6525894 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Primary Mechanism Study of <i>Panax notoginseng</i> Flower (Herb) on Myocardial Infarction in Rats.

Zhou Xin X Li Zhi-Cheng ZC Chen Pei-Pei PP Xie Rui-Fang RF

Cardiology research and practice 20190502

<h4>Background</h4><i>Panax notoginseng</i> (Burk.) F. H. Chen is one of the most common herbs in China. Because of its good efficacy and little adverse reaction, <i>Panax notoginseng</i> has been used widely to treat cardiovascular diseases (CVDs).<h4>Objective</h4>To investigate effects of <i>Panax notoginseng</i> flower (PN-F) on rats with myocardial infarction (MI).<h4>Methods</h4>The proximal left anterior descending coronary artery in rats was ligated to induce acute myocardial infarction. ...[more]

PMID: 31192006

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Project description:BackgroundLiriodendrin is a therapeutic constituent of sargentgloryvine stem which is a famous Chinese traditional medicine. Previous studies have suggested liriodendrin could inhibit different pathways to treat inflammation in lung and intestinal tract. But whether it can treat myocardial infarction (MI) is unknown. We investigated the protective effect of liriodendrin on acute MI in rats and explored the specific mechanisms to expand the use of this traditional Chinese medicine.MethodsThe rats were randomized into the sham group (sham operation), control group (ligation of the left anterior descending artery), and liriodendrin group. The liriodendrin group was intragastrically administered with a liriodendrin solution (100 mg/kg). The control group and the sham group were intragastrically administered with normal saline. Before all rats were euthanized, echocardiography was used to detect their cardiac function. Hematoxylin and eosin (HE) staining and the terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) method were performed. Further quantitative detection of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in tissues were also detected. Western Blot and real-time polymerase chain reaction (RT-PCR) were used to detect the apoptosis and nuclear factor kappa-B (NF-κB) pathway in tissues. H9C2 cells were used to detect the related mechanisms in vitro.ResultsEchocardiography showed that, compared to control group, the cardiac function of the liriodendrin group was significantly improved. histopathological staining of the control group showed that the myocardial tissue was severely damaged, and inflammatory cells were infiltrated. Compared to the control group, the apoptosis index of the liriodendrin group was significantly lower (P<0.05). Enzyme-linked immunosorbent assay (ELISA) results showed that the levels of IL-1β and TNF-α in the control group were higher than those in the liriodendrin group (P<0.05). Meanwhile, apoptosis and the NF-κB pathway were inhibited after liriodendrin administration (P<0.05). Moreover, the mRNA transcriptional activity in the control group was also higher than that in the liriodendrin group (P<0.05). Because of the effect of liriodendrin, NF-κB pathway and apoptosis were downregulated in H9C2 cells which were exposed to ischemia-hypoxia.ConclusionsLiriodendrin may protect myocardial cells after myocardial infarction in rats by inhibiting the release of inflammatory factors, activation of the NF-κB pathway, and apoptosis.

Dataset Information

Primary Mechanism Study of Panax notoginseng Flower (Herb) on Myocardial Infarction in Rats.

Publications

Primary Mechanism Study of <i>Panax notoginseng</i> Flower (Herb) on Myocardial Infarction in Rats.

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