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The Netrin-4/Laminin ?1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells.

ABSTRACT: Neuroblastoma (NB) is the most common pediatric extracranial solid tumor. It arises during development of the sympathetic nervous system. Netrin-4 (NTN4), a laminin-related protein, has been proposed as a key factor to target NB metastasis, although there is controversy about its function. Here, we show that NTN4 is broadly expressed in tumor, stroma and blood vessels of NB patient samples. Furthermore, NTN4 was shown to act as a cell adhesion molecule required for the migration induced by Neogenin-1 (NEO1) in SK-N-SH neuroblastoma cells. Therefore, we propose that NTN4, by forming a ternary complex with Laminin ?1 (LM?1) and NEO1, acts as an essential extracellular matrix component, which induces the migration of SK-N-SH cells.

SUBMITTER: Villanueva AA

PROVIDER: S-EPMC6527380 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells.

Villanueva Andrea A AA Puvogel Sofía S Lois Pablo P Muñoz-Palma Ernesto E Ramírez Orellana Manuel M Lubieniecki Fabiana F Casco Claro Fernando F Gallegos Iván I García-Castro Javier J Sanchez-Gomez Pilar P Torres Vicente A VA Palma Verónica V

Cell adhesion & migration 20180830 1

Neuroblastoma (NB) is the most common pediatric extracranial solid tumor. It arises during development of the sympathetic nervous system. Netrin-4 (NTN4), a laminin-related protein, has been proposed as a key factor to target NB metastasis, although there is controversy about its function. Here, we show that NTN4 is broadly expressed in tumor, stroma and blood vessels of NB patient samples. Furthermore, NTN4 was shown to act as a cell adhesion molecule required for the migration induced by Neoge ...[more]

PMID: 30160193

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The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration.

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