Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device.

ABSTRACT: Extracellular vesicles (EV) containing microRNAs (miRNAs) have tremendous potential as biomarkers for the early detection of disease. Here, we present a simple and rapid PCR-free integrated microfluidics platform capable of absolute quantification (<10% uncertainty) of both free-floating miRNAs and EV-miRNAs in plasma with 1?pM detection sensitivity. The assay time is only 30 minutes as opposed to 13?h and requires only ~20??L of sample as oppose to 1?mL for conventional RT-qPCR techniques. The platform integrates a surface acoustic wave (SAW) EV lysing microfluidic chip with a concentration and sensing microfluidic chip incorporating an electrokinetic membrane sensor that is based on non-equilibrium ionic currents. Unlike conventional RT-qPCR methods, this technology does not require EV extraction, RNA purification, reverse transcription, or amplification. This platform can be easily extended for other RNA and DNA targets of interest, thus providing a viable screening tool for early disease diagnosis, prognosis, and monitoring of therapeutic response.

SUBMITTER: Ramshani Z 

PROVIDER: S-EPMC6527557 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature