Ontology highlight
ABSTRACT:
SUBMITTER: Fernandez-Chacon M
PROVIDER: S-EPMC6531465 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Fernández-Chacón Macarena M Casquero-García Verónica V Luo Wen W Francesca Lunella Federica F Ferreira Rocha Susana S Del Olmo-Cabrera Sergio S Benedito Rui R
Nature communications 20190522 1
Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and a ...[more]