Unknown

Dataset Information

0

Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus.


ABSTRACT: BACKGROUND:Nuclear lamins are type V intermediate filament proteins that maintain nuclear structure and function. Furthermore, Emerin - an interactor of Lamin A/C, facilitates crosstalk between the cytoskeleton and the nucleus as it also interacts with actin and Nuclear Myosin 1 (NM1). RESULTS:Here we show that the depletion of Lamin A/C or Emerin, alters the localization of the nuclear motor protein - Nuclear Myosin 1 (NM1) that manifests as an increase in NM1 foci in the nucleus and are rescued to basal levels upon the combined knockdown of Lamin A/C and Emerin. Furthermore, Lamin A/C-Emerin co-depletion destabilizes cytoskeletal organization as it increases actin stress fibers. This further impinges on nuclear organization, as it enhances chromatin mobility more toward the nuclear interior in Lamin A/C-Emerin co-depleted cells. This enhanced chromatin mobility was restored to basal levels either upon inhibition of Nuclear Myosin 1 (NM1) activity or actin depolymerization. In addition, the combined loss of Lamin A/C and Emerin alters the otherwise highly conserved spatial positions of chromosome territories. Furthermore, knockdown of Lamin A/C or Lamin A/C-Emerin combined, deregulates expression levels of a candidate subset of genes. Amongst these genes, both KLK10 (Chr.19, Lamina Associated Domain (LAD+)) and MADH2 (Chr.18, LAD-) were significantly repressed, while BCL2L12 (Chr.19, LAD-) is de-repressed. These genes differentially reposition with respect to the nuclear envelope. CONCLUSIONS:Taken together, these studies underscore a remarkable interplay between Lamin A/C and Emerin in modulating cytoskeletal organization of actin and NM1 that impinges on chromatin dynamics and function in the interphase nucleus.

SUBMITTER: Ranade D 

PROVIDER: S-EPMC6532135 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus.

Ranade Devika D   Pradhan Roopali R   Jayakrishnan Muhunden M   Hegde Sushmitha S   Sengupta Kundan K  

BMC molecular and cell biology 20190522 1


<h4>Background</h4>Nuclear lamins are type V intermediate filament proteins that maintain nuclear structure and function. Furthermore, Emerin - an interactor of Lamin A/C, facilitates crosstalk between the cytoskeleton and the nucleus as it also interacts with actin and Nuclear Myosin 1 (NM1).<h4>Results</h4>Here we show that the depletion of Lamin A/C or Emerin, alters the localization of the nuclear motor protein - Nuclear Myosin 1 (NM1) that manifests as an increase in NM1 foci in the nucleus  ...[more]

Similar Datasets

| S-EPMC5371638 | biostudies-literature
| S-EPMC6292649 | biostudies-literature
| S-EPMC3927828 | biostudies-literature
| S-EPMC2656162 | biostudies-literature
| S-EPMC3666313 | biostudies-literature
| S-EPMC3785772 | biostudies-literature
| S-EPMC10114975 | biostudies-literature
| S-EPMC6223205 | biostudies-literature
| S-EPMC4058112 | biostudies-literature
| S-EPMC5749162 | biostudies-literature