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A photocleavable surfactant for top-down proteomics.

ABSTRACT: We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.

SUBMITTER: Brown KA

PROVIDER: S-EPMC6532422 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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A photocleavable surfactant for top-down proteomics.

Brown Kyle A KA Chen Bifan B Guardado-Alvarez Tania M TM Lin Ziqing Z Hwang Leekyoung L Ayaz-Guner Serife S Jin Song S Ge Ying Y

Nature methods 20190415 5

We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo i ...[more]

PMID: 30988469

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