Ontology highlight
ABSTRACT:
SUBMITTER: Xiao Y
PROVIDER: S-EPMC6537108 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Xiao Yibei Y Luo Min M Dolan Adam E AE Liao Maofu M Ke Ailong A
Science (New York, N.Y.) 20180607 6397
Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA by the RNA-guided Cascade (CRISPR associated complex for antiviral defense) complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here, we present a 3.7-angstrom-resolution cryo-electron microscopy (cryo-EM) structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop-formin ...[more]