Ontology highlight
ABSTRACT:
SUBMITTER: Huo Y
PROVIDER: S-EPMC4156918 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Nature structural & molecular biology 20140817 9
CRISPR drives prokaryotic adaptation to invasive nucleic acids such as phages and plasmids, using an RNA-mediated interference mechanism. Interference in type I CRISPR-Cas systems requires a targeting Cascade complex and a degradation machine, Cas3, which contains both nuclease and helicase activities. Here we report the crystal structures of Thermobifida fusca Cas3 bound to single-stranded (ss) DNA substrate and show that it is an obligate 3'-to-5' ssDNase that preferentially accepts substrate ...[more]