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Development of a High-Throughput Cytometric Screen to Identify Anti- Wolbachia Compounds: The Power of Public-Private Partnership.


ABSTRACT: The Anti- Wolbachia (A·WOL) consortium at the Liverpool School of Tropical Medicine (LSTM) has partnered with the Global High-Throughput Screening (HTS) Centre at AstraZeneca to create the first anthelmintic HTS for neglected tropical diseases (NTDs). The A·WOL consortium aims to identify novel macrofilaricidal drugs targeting the essential bacterial symbiont ( Wolbachia) of the filarial nematodes causing onchocerciasis and lymphatic filariasis. Working in collaboration, we have validated a robust high-throughput assay capable of identifying compounds that selectively kill Wolbachia over the host insect cell. We describe the development and validation process of this complex, phenotypic high-throughput assay and provide an overview of the primary outputs from screening the AstraZeneca library of 1.3 million compounds.

SUBMITTER: Clare RH 

PROVIDER: S-EPMC6537165 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Development of a High-Throughput Cytometric Screen to Identify Anti- Wolbachia Compounds: The Power of Public-Private Partnership.

Clare Rachel H RH   Clark Roger R   Bardelle Catherine C   Harper Paul P   Collier Matthew M   Johnston Kelly L KL   Plant Helen H   Plant Darren D   McCall Eileen E   Slatko Barton E BE   Cantin Lindsey L   Wu Bo B   Ford Louise L   Murray David D   Rich Kirsty K   Wigglesworth Mark M   Taylor Mark J MJ   Ward Stephen A SA  

SLAS discovery : advancing life sciences R & D 20190408 5


The Anti- Wolbachia (A·WOL) consortium at the Liverpool School of Tropical Medicine (LSTM) has partnered with the Global High-Throughput Screening (HTS) Centre at AstraZeneca to create the first anthelmintic HTS for neglected tropical diseases (NTDs). The A·WOL consortium aims to identify novel macrofilaricidal drugs targeting the essential bacterial symbiont ( Wolbachia) of the filarial nematodes causing onchocerciasis and lymphatic filariasis. Working in collaboration, we have validated a robu  ...[more]

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