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Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.

ABSTRACT: Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.

SUBMITTER: Romei MG

PROVIDER: S-EPMC6537611 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.

Romei Matthew G MG Boxer Steven G SG

Annual review of biophysics 20190220

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies. ...[more]

PMID: 30786230

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