Ontology highlight
ABSTRACT:
SUBMITTER: Akat KM
PROVIDER: S-EPMC6538341 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Akat Kemal M KM Lee Youngmin A YA Hurley Arlene A Morozov Pavel P Max Klaas Ea KE Brown Miguel M Bogardus Kimberly K Sopeyin Anuoluwapo A Hildner Kai K Diacovo Thomas G TG Neurath Markus F MF Borggrefe Martin M Tuschl Thomas T
JCI insight 20190411
Extracellular mRNAs (ex-mRNAs) potentially supersede extracellular miRNAs (ex-miRNAs) and other RNA classes as biomarkers. We performed conventional small-RNA-sequencing (sRNA-seq) and sRNA-seq with T4 polynucleotide kinase (PNK) end-treatment of total exRNA isolated from serum and platelet-poor EDTA, ACD, and heparin plasma to study the effect on ex-mRNA capture. Compared to conventional sRNA-seq PNK-treatment increased the detection of informative ex-mRNAs reads up to 50-fold. The exRNA pool w ...[more]