Ontology highlight
ABSTRACT:
SUBMITTER: Hirvonen LM
PROVIDER: S-EPMC6538533 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Methods and applications in fluorescence 20180709 4
Superresolution microscopy based on localisation is usually performed in a buffer containing enzymatic oxygen scavenger, which facilitates reversible photoswitching of the dye molecules. This makes correlative fluorescence localisation and atomic force microscopy (AFM) challenging, because enzymatic oxygen scavenging interferes with the AFM cantilevers. Here we report on the blinking kinetics of a new red cyanine dye, iFluor-647, which is similar to the Alexa-647 dye commonly used for superresol ...[more]