Nanomechanical Induction of Autophagy-Related Fluorescence in Single Cells with Atomic Force Microscopy.

ABSTRACT: Mechanistic understanding of how living systems sense, transduce, and respond to mechanical cues has important implications in development, physiology, and therapy. Here, the authors use an integrated atomic force microscope (AFM) and brightfield/epifluorescent microscope platform to precisely simulate living single cells or groups of cells under physiological conditions, in real time, concomitantly measuring the single-cell autophagic response and its transmission to neighboring cells. Dual-color fluorescence monitoring of the cellular autophagic response reveals the dynamics of autophagosome formation, degradation, and induction in neighboring contacting and noncontacting cells. Autophagosome formation is dependent on both the applied force and contact area of the AFM tip. More important

SUBMITTER: Li B 

PROVIDER: S-EPMC8693060 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature