Neuronal methylome reveals CREB-associated neuro-axonal impairment in multiple sclerosis.

ABSTRACT:

Background

Due to limited access to brain tissue, the precise mechanisms underlying neuro-axonal dysfunction in neurological disorders such as multiple sclerosis (MS) are largely unknown. In that context, profiling DNA methylation, which is a stable and cell type-specific regulatory epigenetic mark of genome activity, offers a unique opportunity to characterize the molecular mechanisms underpinning brain pathology in situ. We examined DNA methylation patterns of neuronal nuclei isolated from post-mortem brain tissue to infer processes that occur in neurons of MS patients.

Results

We isolated subcortical neuronal nuclei from post-mortem white matter tissue of MS patients and non-neurological controls using flow cytometry. We examined bulk DNA methylation changes (total n = 29

SUBMITTER: Kular L 

PROVIDER: S-EPMC6543588 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature