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Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.

ABSTRACT: Photosynthetic phenotyping requires quick characterization of dynamic traits when measuring large plant numbers in a fluctuating environment. Here, we evaluated the light-induced fluorescence transient (LIFT) method for its capacity to yield rapidly fluorometric parameters from 0.6 m distance. The close approximation of LIFT to conventional chlorophyll fluorescence (ChlF) parameters is shown under controlled conditions in spinach leaves and isolated thylakoids when electron transport was impaired by anoxic conditions or chemical inhibitors. The ChlF rise from minimum fluorescence (F_o) to maximum fluorescence induced by fast repetition rate (F_m-FRR) flashes was dominated by reduction of the primary electron acceptor in photosystem II (Q_A). The subsequent reoxidation of Q_A^- was quantified using the relaxation of ChlF in 0.65 ms (F_r1) and 120 ms (F_r2) phases. Reoxidation efficiency of Q_A^- (F_r1/F_v, where F_v = F_m-FRR?-?F_o) decreased when electron transport was impaired, while quantum efficiency of photosystem II (F_v/F_m) showed often no significant effect. ChlF relaxations of the LIFT were similar to an independent other method. Under increasing light intensities, F_r2'/F_q' (where F_r2' and F_q' represent F_r2 and F_v in the light-adapted state, respectively) was hardly affected, whereas the operating efficiency of photosystem II (F_q'/F_m') decreased due to non-photochemical quenching. F_m-FRR was significantly lower than the ChlF maximum induced by multiple turnover (F_m-MT) flashes. However, the resulting F_v/F_m and F_q'/F_m' from both flashes were highly correlated. The LIFT method complements F_v/F_m with information about efficiency of electron transport. Measurements in situ and from a distance facilitate application in high-throughput and automated phenotyping.

SUBMITTER: Keller B

PROVIDER: S-EPMC6548062 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.

Keller Beat B Vass Imre I Matsubara Shizue S Paul Kenny K Jedmowski Christoph C Pieruschka Roland R Nedbal Ladislav L Rascher Uwe U Muller Onno O

Photosynthesis research 20181024 2

Photosynthetic phenotyping requires quick characterization of dynamic traits when measuring large plant numbers in a fluctuating environment. Here, we evaluated the light-induced fluorescence transient (LIFT) method for its capacity to yield rapidly fluorometric parameters from 0.6 m distance. The close approximation of LIFT to conventional chlorophyll fluorescence (ChlF) parameters is shown under controlled conditions in spinach leaves and isolated thylakoids when electron transport was impaire ...[more]

PMID: 30357678

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Project description:BackgroundOver the past decades, the structural and functional genomics of rice have been deeply studied, and high density of molecular genetic markers have been developed. However, the genetic variation in leaf photosynthesis, the most important trait for rice yield improvement, was rarely studied. The lack of photosynthesis phenotyping tools is one of the bottlenecks, as traditional direct photosynthesis measurements are very low-throughput, and recently developed high-throughput methods are indirect measurements. Hence, there is an urgent need for a fast, accurate and direct measurement approach.ResultWe reported a fast photosynthesis measurement (FPM) method for phenotyping photosynthetic capacity of rice, which measures photosynthesis of excised tillers in environment-controlled lab conditions. The light response curves measured using FPM approach coped well with that the curves measured using traditional gas exchange approach. Importantly, the FPM technique achieved an average throughput of 5.4 light response curves per hour, which was 3 times faster than the 1.8 light response curves per hour using the traditional method. Tillers sampled at early morning had the highest photosynthesis, stomatal conductance and the lowest variability. In addition, even 12 h after sampling, there was no significant difference of photosynthesis rate between excised tillers and in situ. We finally investigated the genetic variations of photosynthetic traits across 568 F2 lines using the FPM technique and discussed the logistics of screening several hundred samples per day per instrumental unit using FPM to generate a wealth of photosynthetic phenotypic data, which might help to improve the selection power in large populations of rice with the ultimate aim of improving yield through improved photosynthesis.ConclusionsHere we developed a high-throughput method that can measure the rice leaf photosynthetic capacity approximately 10 times faster than traditional gas exchange approaches. Importantly, this method can overcome measurement errors caused by environmental heterogeneity under field conditions, and it is possible to measure 12 or more hours per day under lab conditions.

Dataset Information

Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.

Publications

Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.

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