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ABSTRACT:
SUBMITTER: Litke JL
PROVIDER: S-EPMC6554452 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Litke Jacob L JL Jaffrey Samie R SR
Nature biotechnology 20190408 6
RNA aptamers and RNA aptamer-based devices can be genetically encoded and expressed in cells to probe and manipulate cellular function. However, their usefulness in the mammalian cell is limited by low expression and rapid degradation. Here we describe the Tornado (Twister-optimized RNA for durable overexpression) expression system for achieving rapid RNA circularization, resulting in RNA aptamers with high stability and expression levels. Tornado-expressed transcripts contain an RNA of interest ...[more]