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The in vitro effects of gibberellin on human sperm motility.


ABSTRACT: Gibberellin, a plant growth regulator, is widely used to increase the shelf life and quality of fruits and vegetables. In this study, human semen samples were exposed to different concentrations of gibberellin, which reduced spermatozoa motility in vitro. Gibberellin exposure also increased levels of reactive oxygen species and the protein levels of apoptosis markers in human sperm. Gibberellin inhibited the activity of Na+/K+-adenosine triphosphatase (ATPase) and Ca2+-ATPase, which maintain the stability of ions inside and outside the membranes of spermatozoa. Moreover, gibberellin exposure suppressed adenosine triphosphate production and reduced the protein levels of adenosine triphosphate synthases, which may have induced the protein expression of adenosine 5'-monophosphate-activated protein kinase (AMPK) and its phosphorylated form. These results suggest that gibberellin reduces human sperm motility in vitro by increasing reactive oxygen species levels and reducing ATPase activity, which may upregulate AMPK and consequently reduce the fertilization potential of spermatozoa.

SUBMITTER: Xu CS 

PROVIDER: S-EPMC6555458 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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The <i>in vitro</i> effects of gibberellin on human sperm motility.

Xu Chun-Shuang CS   Zhou Yi Y   Jiang Zhou Z   Wang Li-E LE   Huang Jiao-Jiao JJ   Zhang Tian-Yu TY   Zhao Yong Y   Shen Wei W   Zou Shu-Hua SH   Zang Li-Li LL  

Aging 20190501 10


Gibberellin, a plant growth regulator, is widely used to increase the shelf life and quality of fruits and vegetables. In this study, human semen samples were exposed to different concentrations of gibberellin, which reduced spermatozoa motility <i>in vitro</i>. Gibberellin exposure also increased levels of reactive oxygen species and the protein levels of apoptosis markers in human sperm. Gibberellin inhibited the activity of Na<sup>+</sup>/K<sup>+</sup>-adenosine triphosphatase (ATPase) and Ca  ...[more]

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