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Developing chemically modified redox-responsive proteins as smart therapeutics.

ABSTRACT: The conditional control of protein function in response to the physiological changes of diseased cells is essential to develop smart protein therapeutics. Herein, we report a redox-responsive chemical modification of a protein by conjugating an intracellular glutathione (GSH)-cleavable ligand, NSA, onto a protein residue. We demonstrated that the NSA conjugation of Ribonuclease A (RNase A) enabled the control of the protein function by GSH in an aqueous solution and living cells, with extended applications for targeted cancer therapy using a lipid nanoparticle-based intracellular protein delivery strategy.

SUBMITTER: Tang Q

PROVIDER: S-EPMC6556230 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Developing chemically modified redox-responsive proteins as smart therapeutics.

Tang Qiao Q Wang Jianxue J Jiang Ying Y Zhang Meining M Chang Jin J Xu Qiaobing Q Mao Lanqun L Wang Ming M

Chemical communications (Cambridge, England) 20190401 35

The conditional control of protein function in response to the physiological changes of diseased cells is essential to develop smart protein therapeutics. Herein, we report a redox-responsive chemical modification of a protein by conjugating an intracellular glutathione (GSH)-cleavable ligand, NSA, onto a protein residue. We demonstrated that the NSA conjugation of Ribonuclease A (RNase A) enabled the control of the protein function by GSH in an aqueous solution and living cells, with extended a ...[more]

PMID: 30984934

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