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Effect of Ocular Hypertension on D-?-Aspartic Acid-Containing Proteins in the Retinas of Rats.

ABSTRACT: Purpose:To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins. Methods:Adult Wistar rats with ocular hypertension were used as an experimental model. D-?-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-?-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA. Results:D-?-aspartic acid was expressed in a protein band at around 44.5?kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5?kDa protein band was mainly composed of ?-enolase, S-arrestin, and ATP synthase subunits ? and ?, in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats. Conclusion:Ocular hypertension affected the expression of proteins containing D-?-aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.

SUBMITTER: Kanamoto T 

PROVIDER: S-EPMC6556240 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Effect of Ocular Hypertension on D-<i>β</i>-Aspartic Acid-Containing Proteins in the Retinas of Rats.

Kanamoto Takashi T   Tachibana Takashi T   Kitaoka Yasushi Y   Hisatomi Toshio T   Ikeda Yasuhiro Y   Murakami Yusuke Y   Tobiume Kei K   Asaoka Ryo R   Kiuchi Yoshiaki Y  

Journal of ophthalmology 20190521

<h4>Purpose</h4>To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins.<h4>Methods</h4>Adult Wistar rats with ocular hypertension were used as an experimental model. D-<i>β</i>-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-<i>β</i>-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA.<h4>Results</h4>D-<i>β</i>-aspa  ...[more]

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