Screening and analysis of differentially expressed genes of human melanocytes in skin cells mixed culture.

ABSTRACT:

Background

This study aims to screen the key genes and possible signaling pathways involved in the differentiation and proliferation of human melanocytes (MCs) by in vitro culture of mixed skin cells. This will be helpful to further study the mechanisms and treatment strategies of pigment-related diseases such as vitiligo.

Methods

Mixed skin cells were obtained by digesting and separating normal human foreskin tissues. Ribonucleic acid (RNA) was extracted from sorting cells and high-throughput transcriptome sequencing was performed at different culture time points. Differentially expressed genes (DEGs) were obtained by comparing the expression abundance of genes at different culture time points. Then the key genes and signaling pathways involved in the differentiation and pr

SUBMITTER: Mei X 

PROVIDER: S-EPMC6556658 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature