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Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.

ABSTRACT: We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.

SUBMITTER: Yang B

PROVIDER: S-EPMC6557432 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.

Yang Bin B Chen Xingye X Wang Yina Y Feng Siyu S Pessino Veronica V Stuurman Nico N Cho Nathan H NH Cheng Karen W KW Lord Samuel J SJ Xu Linfeng L Xu Linfeng L Xie Dan D Mullins R Dyche RD Leonetti Manuel D MD Huang Bo B

Nature methods 20190506 6

We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording ...[more]

PMID: 31061492

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