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Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.

ABSTRACT: We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

SUBMITTER: Abrahamsson S

PROVIDER: S-EPMC5611928 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.

Abrahamsson Sara S Blom Hans H Agostinho Ana A Jans Daniel C DC Jost Aurelie A Müller Marcel M Nilsson Linnea L Bernhem Kristoffer K Lambert Talley J TJ Heintzmann Rainer R Brismar Hjalmar H

Biomedical optics express 20170817 9

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging cap ...[more]

PMID: 28966852

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