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GPR124 regulates microtubule assembly, mitotic progression, and glioblastoma cell proliferation.

ABSTRACT: GPR124 is involved in embryonic development and remains expressed by select organs. The importance of GPR124 during development suggests that its aberrant expression might participate in tumor growth. Here we show that both increases and decreases in GPR124 expression in glioblastoma cells reduce cell proliferation by differentially altering the duration mitotic progression. Using mass spectrometry-based proteomics, we discovered that GPR124 interacts with ch-TOG, a known regulator of both microtubule (MT)-plus-end assembly and mitotic progression. Accordingly, changes in GPR124 expression and ch-TOG similarly affect MT assembly measured by real-time microscopy in cells. Our study describes a novel molecular interaction involving GPR124 and ch-TOG at the plasma membrane that controls glioblastoma cell proliferation by modifying MT assembly rates and controlling the progression of distinct phases of mitosis.

SUBMITTER: Cherry AE

PROVIDER: S-EPMC6557680 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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GPR124 regulates microtubule assembly, mitotic progression, and glioblastoma cell proliferation.

Cherry Allison E AE Vicente Juan Jesus JJ Xu Cong C Morrison Richard S RS Ong Shao-En SE Wordeman Linda L Stella Nephi N

Glia 20190506 8

GPR124 is involved in embryonic development and remains expressed by select organs. The importance of GPR124 during development suggests that its aberrant expression might participate in tumor growth. Here we show that both increases and decreases in GPR124 expression in glioblastoma cells reduce cell proliferation by differentially altering the duration mitotic progression. Using mass spectrometry-based proteomics, we discovered that GPR124 interacts with ch-TOG, a known regulator of both micro ...[more]

PMID: 31058365

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