Ontology highlight
ABSTRACT:
SUBMITTER: Thakur AK
PROVIDER: S-EPMC6557705 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Thakur Avinash Kumar AK Movileanu Liviu L
Nature biotechnology 20181210
Protein-protein interactions (PPIs) are essential for many cellular processes. However, transient PPIs are difficult to measure at high throughput or in complex biological fluids using existing methods. We engineered a genetically encoded sensor for real-time sampling of transient PPIs at single-molecule resolution. Our sensor comprises a truncated outer membrane protein pore, a flexible tether, a protein receptor and a peptide adaptor. When a protein ligand present in solution binds to the rece ...[more]