Ontology highlight
ABSTRACT:
SUBMITTER: Zhang B
PROVIDER: S-EPMC6559982 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Nature communications 20190611 1
Cas13d, the type VI-D CRISPR-Cas effector, is an RNA-guided ribonuclease that has been repurposed to edit RNA in a programmable manner. Here we report the detailed structural and functional analysis of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex. Two hydrated Mg<sup>2+</sup> ions aid in stabilizing the conformation of the crRNA repeat region. Sequestration of divalent metal ions does not alter pre-crRNA processing, but abolishes target cleavage by UrCas13d. Notably, the pre-c ...[more]