Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.
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ABSTRACT:
SUBMITTER: Schneider A
PROVIDER: S-EPMC6560178 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Publications
Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.
Schneider Annika A Kurz Sandra S Manske Katrin K Janas Marianne M Heikenwälder Mathias M Misgeld Thomas T Aichler Michaela M Weissmann Sebastian Felix SF Zischka Hans H Knolle Percy P Wohlleber Dirk D
Scientific reports 20190611 1
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during vir ...[more]
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