Unknown

Dataset Information

0

White Adipose Tissue Response of Obese Mice to Ambient Oxygen Restriction at Thermoneutrality: Response Markers Identified, but no WAT Inflammation.


ABSTRACT: Obesity is associated with white adipose tissue (WAT) hypoxia and inflammation. We aimed to test whether mild environmental oxygen restriction (OxR, 13% O2), imposing tissue hypoxia, triggers WAT inflammation in obese mice. Thirteen weeks diet-induced obese male adult C57BL/6JOlaHsd mice housed at thermoneutrality were exposed for five days to OxR versus normoxia. WAT and blood were isolated and used for analysis of metabolites and adipokines, WAT histology and macrophage staining, and WAT transcriptomics. OxR increased circulating levels of haemoglobin and haematocrit as well as hypoxia responsive transcripts in WAT and decreased blood glucose, indicating systemic and tissue hypoxia. WAT aconitase activity was inhibited. Macrophage infiltration as marker for WAT inflammation tended to be decreased, which was supported by down regulation of inflammatory genes S100a8, Ccl8, Clec9a, Saa3, Mgst2, and Saa1. Other down regulated processes include cytoskeleton remodelling and metabolism, while response to hypoxia appeared most prominently up regulated. The adipokines coiled-coil domain containing 3 (CCDC3) and adiponectin, as well as the putative WAT hormone cholecystokinin (CCK), were reduced by OxR on transcript (Cck, Ccdc3) and/or serum protein level (adiponectin, CCDC3). Conclusively, our data demonstrate that also in obese mice OxR does not trigger WAT inflammation. However, OxR does evoke a metabolic response in WAT, with CCDC3 and adiponectin as potential markers for systemic or WAT hypoxia.

SUBMITTER: Hoevenaars FPM 

PROVIDER: S-EPMC6562665 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

White Adipose Tissue Response of Obese Mice to Ambient Oxygen Restriction at Thermoneutrality: Response Markers Identified, but no WAT Inflammation.

Hoevenaars Femke P M FPM   Keijer Jaap J   van der Stelt Inge I   Duivenvoorde Loes P M LPM   Herreman Laure L   van Nes Robin R   Friedecký David D   Hegeman Maria A MA   van Schothorst Evert M EM  

Genes 20190510 5


Obesity is associated with white adipose tissue (WAT) hypoxia and inflammation. We aimed to test whether mild environmental oxygen restriction (OxR, 13% O<sub>2</sub>), imposing tissue hypoxia, triggers WAT inflammation in obese mice. Thirteen weeks diet-induced obese male adult C57BL/6JOlaHsd mice housed at thermoneutrality were exposed for five days to OxR versus normoxia. WAT and blood were isolated and used for analysis of metabolites and adipokines, WAT histology and macrophage staining, an  ...[more]

Similar Datasets

| S-EPMC5362617 | biostudies-literature
2020-07-13 | E-MTAB-9062 | biostudies-arrayexpress
| S-EPMC8385178 | biostudies-literature
2020-09-21 | GSE64238 | GEO
| S-EPMC7099615 | biostudies-literature
| S-EPMC4878191 | biostudies-literature
| PRJEB11666 | ENA
| S-EPMC6071773 | biostudies-literature
| S-EPMC4758873 | biostudies-literature
| S-EPMC7903014 | biostudies-literature