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Simplified Transformation of Ostreococcus tauri Using Polyethylene Glycol.

ABSTRACT: Ostreococcustauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13-22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic data produced from marine samples. Here we describe a simplified and robust transformation protocol for the smallest of these algae (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than the previously described electroporation protocol. Short (2 min or less) incubation times in PEG gave >10⁴ transformants per microgram DNA. The time of cell recovery after transformation could be reduced to a few hours, permitting the experiment to be done in a day rather than overnight as used in previous protocols. DNA was randomly inserted in the O. tauri genome. In our hands PEG was 20-40-fold more efficient than electroporation for the transformation of O. tauri, and this improvement will facilitate mutagenesis of all of the dispensable genes present in the tiny O. tauri genome.

SUBMITTER: Sanchez F

PROVIDER: S-EPMC6562926 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Photoperiodic transcriptional responses in Ostreococcus tauri

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Bacteria in Ostreococcus tauri cultures - friends, foes or hitchhikers?

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Transcriptional response of Ostreococcus tauri to Cu challenge

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2019-05-04 | GSE114058 | GEO

Organellar inheritance in the green lineage: insights from Ostreococcus tauri.

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Cryo-ET of natural chromatin from Ostreococcus tauri and Saccharyomyces cerevisiae

Project description: Not available

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Identification and analysis of OsttaDSP, a phosphoglucan phosphatase from Ostreococcus tauri.

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Marinobacter Dominates the Bacterial Community of the Ostreococcus tauri Phycosphere in Culture.

Project description:Microalgal-bacterial interactions are commonly found in marine environments and are well known in diatom cultures maintained in laboratory. These interactions also exert strong effects on bacterial and algal diversity in the oceans. Small green eukaryote algae of the class Mamiellophyceae (Chlorophyta) are ubiquitous and some species, such as Ostreococcus spp., are particularly important in Mediterranean coastal lagoons, and are observed as dominant species during phytoplankton blooms in open sea. Despite this, little is known about the diversity of bacteria that might facilitate or hinder O. tauri growth. We show, using rDNA 16S sequences, that the bacterial community found in O. tauri RCC4221 laboratory cultures is dominated by γ-proteobacteria from the Marinobacter genus, regardless of the growth phase of O. tauri RCC4221, the photoperiod used, or the nutrient conditions (limited in nitrogen or phosphorous) tested. Several strains of Marinobacter algicola were detected, all closely related to strains found in association with taxonomically distinct organisms, particularly with dinoflagellates and coccolithophorids. These sequences were more distantly related to M. adhaerens, M. aquaeoli and bacteria usually associated to euglenoids. This is the first time, to our knowledge, that distinct Marinobacter strains have been found to be associated with a green alga in culture.

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Permeation properties of a P2X receptor in the green algae Ostreococcus tauri.

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