Ontology highlight
ABSTRACT:
SUBMITTER: Huang WYC
PROVIDER: S-EPMC6563836 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Huang William Y C WYC Alvarez Steven S Kondo Yasushi Y Lee Young Kwang YK Chung Jean K JK Lam Hiu Yue Monatrice HYM Biswas Kabir H KH Kuriyan John J Groves Jay T JT
Science (New York, N.Y.) 20190307 6431
The guanine nucleotide exchange factor (GEF) Son of Sevenless (SOS) is a key Ras activator that is autoinhibited in the cytosol and activates upon membrane recruitment. Autoinhibition release involves structural rearrangements of the protein at the membrane and thus introduces a delay between initial recruitment and activation. In this study, we designed a single-molecule assay to resolve the time between initial receptor-mediated membrane recruitment and the initiation of GEF activity of indivi ...[more]