Ontology highlight
ABSTRACT:
SUBMITTER: Allodi I
PROVIDER: S-EPMC6565614 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Allodi Ilary I Nijssen Jik J Benitez Julio Aguila JA Schweingruber Christoph C Fuchs Andrea A Bonvicini Gillian G Cao Ming M Kiehn Ole O Hedlund Eva E
Stem cell reports 20190509 6
Oculomotor neurons, which regulate eye movement, are resilient to degeneration in the lethal motor neuron disease amyotrophic lateral sclerosis (ALS). It would be highly advantageous if motor neuron resilience could be modeled in vitro. Toward this goal, we generated a high proportion of oculomotor neurons from mouse embryonic stem cells through temporal overexpression of PHOX2A in neuronal progenitors. We demonstrate, using electrophysiology, immunocytochemistry, and RNA sequencing, that in vit ...[more]