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The cryo-EM method microcrystal electron diffraction (MicroED).

ABSTRACT: In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.

SUBMITTER: Nannenga BL

PROVIDER: S-EPMC6568260 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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The cryo-EM method microcrystal electron diffraction (MicroED).

Nannenga Brent L BL Gonen Tamir T

Nature methods 20190429 5

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospe ...[more]

PMID: 31040436

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