Ontology highlight
ABSTRACT:
SUBMITTER: Guacci V
PROVIDER: S-EPMC6579514 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
eLife 20190604
Cohesin mediates higher order chromosome structure. Its biological activities require topological entrapment of DNA within a lumen(s) formed by cohesin subunits. The reversible dissociation of cohesin's Smc3p and Mcd1p subunits is postulated to form a regulated gate that allows DNA entry and exit into the lumen. We assessed gate-independent functions of this interface in yeast using a fusion protein that joins Smc3p to Mcd1p. We show that in vivo all the regulators of cohesin promote DNA binding ...[more]