Dataset Information

MiR?342 inhibits glioma cell proliferation by targeting GPRC5A.

ABSTRACT: Accumulating evidence suggests that microRNAs (miRNAs) play a key role in the biological behaviors and progression of glioma. However, the function and bio?molecular mechanisms of miR?342 in glioma remain largely unclear. In the present study, reverse transcription quantitative?polymerase chain reaction and western blotting were performed to determine the mRNA and protein expression levels of the factors investigated. MTT assay was performed to examine the proliferation rates. Luciferase reporter assay was performed to test the binding between miRNA?342 and its putative target. Data indicated that miR?342 expression was markedly decreased in human glioma tissues and cell line U87, and reduced miR?342 expression significantly promoted cell proliferation. In order to explore the mechanisms, G?protein coupled receptor family C group 5 member A (GPRC5A) was identified as a target of miR?342 and depletion of GPRC5A suppressed cell proliferation. Our findings demonstrated that miR?342 regulates the cell proliferation of glioma by targeting GPRC5A, which indicates that miR?342 is a target of interest regarding the treatment of refractory glioma, and it may provide a promising prognostic and therapeutic strategy for glioma treatment.

SUBMITTER: Wang J

PROVIDER: S-EPMC6579993 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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miR‑342 inhibits glioma cell proliferation by targeting GPRC5A.

Wang Jianjiao J Yang Yan Y Cao Yuandong Y Tang Xinyu X

Molecular medicine reports 20190515 1

Accumulating evidence suggests that microRNAs (miRNAs) play a key role in the biological behaviors and progression of glioma. However, the function and bio‑molecular mechanisms of miR‑342 in glioma remain largely unclear. In the present study, reverse transcription quantitative‑polymerase chain reaction and western blotting were performed to determine the mRNA and protein expression levels of the factors investigated. MTT assay was performed to examine the proliferation rates. Luciferase reporte ...[more]

PMID: 31115523

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Project description:Glioma is an extremely aggressive malignant neoplasm of the central nervous system. MicroRNA (miRNA) are known to bind to specific target mRNA to regulate post-transcriptional gene expression and are, therefore, currently regarded as promising biomarkers for glioma diagnosis and prognosis. The aim of the present study was to examine the pathogenesis and potential molecular markers of glioma by comparing the differential expression of miRNA and mRNA between glioma tissue and peritumor brain tissue. We explored the impact of screened core miRNA and mRNA on cell proliferation, invasion, and migration of glioma. An miRNA expression profile dataset (GSE90603) and a transcriptome profile dataset (GSE90598) were downloaded from combined miRNA-mRNA microarray chips in the Gene Expression Omnibus (GEO) database. Overall, 59 differentially expressed miRNAs (DEMs) and 419 differentially expressed genes (DEGs) were identified using the R limma software package. FunRich software was used to predict DEM target genes and miRNA-gene pairs, and Perl software was used to find overlapping genes between DEGs and DEM target genes. There were 129 overlapping genes regulated by nine miRNAs between target genes of the DEMs and DEGs. The Chinese Glioma Genome Atlas(CGGA) was analyzed in order to identify miRNAs with diagnostic and prognostic significance. MiR-139-5p, miR-137, and miR-338-3p were validated to be significantly linked to prognosis in glioma patients. Finally, we validated that miR-139-5p affected glioma malignant biological behavior via targeting gamma-aminobutyric acid A receptor alpha 1(GABRA1) through rescue experiments. Low miR-139-5p expression was correlated with survival probability and World Health Organization (WHO) grade. MiR-139-5p overexpression inhibited cell proliferation, migration, and invasion of glioma in vitro. GABRA1 was identified as a functional downstream target of miR-139-5p. Decreased GABRA1 expression was related to similar biological roles as miR-139-5p overexpression while upregulation of GABRA1 effectively reversed the inhibition effects of miR-139-5p. These results demonstrate a novel axis for miR-139-5p/GABRA1 in glioma progression and provide potential prognostic predictors and therapeutic target for glioma patients.

Dataset Information

MiR?342 inhibits glioma cell proliferation by targeting GPRC5A.

Publications

miR‑342 inhibits glioma cell proliferation by targeting GPRC5A.

Similar Datasets

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