Dataset Information

Microsatellite markers for Saussurea polylepis (Asteraceae), a vulnerable continental island species endemic to Korea.

ABSTRACT: Premise:Nuclear microsatellite markers were developed for Saussurea polylepis (Asteraceae), a vulnerable species with very limited distribution in a few southwestern continental islands of the Korean peninsula, in order to facilitate future population genetic studies. Methods and Results:Based on the Illumina sequence data, a total of 21 microsatellite primer pairs were designed and tested for their suitability. Nineteen of these primers, with two to 11 alleles per locus, were polymorphic in three natural populations of S. polylepis. The levels of expected and observed heterozygosity ranged from 0.000 to 0.842 and 0.000 to 0.933, respectively. Sixteen of these simple sequence repeat (SSR) markers were successfully cross-amplified in five congeneric species, namely S. gracilis, S. grandifolia, and S. tanakae for all 21 loci, and S. maximowiczii and S. pulchella for 18 loci. Conclusions:The SSR markers developed here will be useful for future population genetic studies on S. polylepis and related species.

SUBMITTER: Yun SA

PROVIDER: S-EPMC6580984 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Publications

Microsatellite markers for <i>Saussurea polylepis</i> (Asteraceae), a vulnerable continental island species endemic to Korea.

Yun Seon A SA Kim Seung-Chul SC

Applications in plant sciences 20190614 6

<h4>Premise</h4>Nuclear microsatellite markers were developed for <i>Saussurea polylepis</i> (Asteraceae), a vulnerable species with very limited distribution in a few southwestern continental islands of the Korean peninsula, in order to facilitate future population genetic studies.<h4>Methods and results</h4>Based on the Illumina sequence data, a total of 21 microsatellite primer pairs were designed and tested for their suitability. Nineteen of these primers, with two to 11 alleles per locus, w ...[more]

PMID: 31236317

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Project description:Saussurea polylepis Nakai is an herbaceous perennial endemic to Korea and is highly restricted to several continental islands in the southwestern part of the Korean Peninsula. Given its very narrow geographical distribution, it is more vulnerable to anthropogenic activities and global climate changes than more widely distributed species. Despite the need for comprehensive genetic information for conservation and management, no such population genetic studies of S. polylepis have been conducted. In this study, genetic diversity and population structure were evaluated for 97 individuals from 5 populations (Gwanmaedo, Gageodo, Hongdo, Heusando, and Uido) using 19 polymorphic microsatellites. The populations were separated by a distance of 20-90 km. We found moderate levels of genetic diversity in S. polylepis (Ho = 0.42, He = 0.43). This may be due to long lifespans, outcrossing, and gene flow, despite its narrow range. High levels of gene flow (Nm = 1.76, mean Fst = 0.09), especially from wind-dispersed seeds, would contribute to low levels of genetic differentiation among populations. However, the small population size and reduced number of individuals in the reproductive phase of S. polylepis can be a major threat leading to inbreeding depression and genetic diversity loss. Bayesian cluster analysis revealed three significant structures at K = 3, consistent with DAPC and UPGMA. It is thought that sea level rise after the last glacial maximum may have acted as a geographical barrier, limiting the gene flow that would lead to distinct population structures. We proposed the Heuksando population, which is the largest island inhabited by S. polylepis, as a source population because of its large population size and high genetic diversity. Four management units (Gwanmaedo, Gageodo, Hongdo-Heuksando, and Uido) were suggested for conservation considering population size, genetic diversity, population structure, unique alleles, and geographical location (e.g., proximity).

Dataset Information

Microsatellite markers for Saussurea polylepis (Asteraceae), a vulnerable continental island species endemic to Korea.

Publications

Microsatellite markers for <i>Saussurea polylepis</i> (Asteraceae), a vulnerable continental island species endemic to Korea.

Similar Datasets

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